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MHC Class II Antibody
SPA-07572
| Size | Price |
| 0.5 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | MHC Class II |
| Gene Abbr. | HLA-DMA |
| Gene ID | 3108 |
| Full Name | major histocompatibility complex, class II, DM alpha |
| Alias | D6S222E, DMA, HLADM, RING6 |
| Introduction | MHC Class II antigens are heterodimers consisting of one alpha chain(31-34kD) and one beta chain (26-29kD). The family of monoclonal antibodies (ER-TR 3, ER-TR 2, ER-TR 1) detect MHC class II antigens encoded by the murine Ia region of the H-2 complex, corresponding to the human HLA-DR region. MHC Class II antigens are a valuable tool for studying T helper cell interaction with class II positive antigen presenting cells (dendritic cells, B cells, macrophages) and offer new possibilities for studying the development of T helper cells since these antibodies also stain stromal cells in the thymus. MHC Class II antigens are also inducible on a number of other cells (endothelium and epithelial cells) by interferon gamma. |
| Product Details | |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Clone No. | M5/114 |
| Isotype | IgG2B |
| Immunogen | Activated C57BL/6 mouse spleen cells. |
| Usage | |
|---|---|
| Application | WB, FC, IHC, IP |
| Dilutions | Western Blot (1:500-1:2000); Flow Cytometry (2 µg/mL); Immunohistochemistry (1:50-1:300); Immunoprecipitation (1:10-1:500) |
| Reactivity | Mouse |
| Specificity | The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes. |
| Storage & Handling | |
|---|---|
| Storage Buffer | PBS (pH 7.4), 0.2 µm filter sterilized. |
| Preservative | No Preservative |
| Storage Temp. | Store at 4 °C. |
| Handling | Do not freeze. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.