Methyl-Histone H3 Antibody - CD BioSciences

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Methyl-Histone H3 Antibody

Methyl-Histone H3 Antibody

SPA-05126

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100 µg Online Inquiry
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Target Information
Target Name Histone H3
Gene Abbr. H3-3A
Gene ID 3020
Full Name H3.3 histone A
Alias H3-3B, H3.3A, H3F3, H3F3A
Introduction Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic methylated peptide corresponding to residues surrounding Arg2 of human histone H3.
Source/Purification Antibodies are produced by immunizing animals with a synthetic methylated peptide corresponding to residues surrounding Arg2 of human histone H3. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 17
Reactivity Human
Sensitivity Endogenous
Specificity It detects endogenous levels of histone H3 only when mono- and di-methylated at arginine 2. The antibody does not cross-react with other histones.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

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