MEK1 Antibody - CD BioSciences

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MEK1 Antibody

MEK1 Antibody

SPA-07393

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Target Information
Target Name MEK1
Gene Abbr. MAP2K1
Gene ID 5604
Full Name mitogen-activated protein kinase kinase 1
Alias CFC3, MAPKK1, MEK1, MEL, MKK1
Introduction MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx. Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells. MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 239421
Isotype IgG2A
Immunogen E. coli-derived recombinant human MEK1. Lys2-Val393 (Ser218Asp, Ser222Asp). Q02750.
Usage
Application WB
Dilutions Western Blot (0.5 µg/mL)
MW(KDa) 45
Reactivity Human, Mouse, Rat
Specificity Detects human, mouse, and rat MEK1 in Western Blots. In Western Blots, no crossreactivity with recombinant human MEK2 is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.