MEK1 Antibody - CD BioSciences

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MEK1 Antibody

MEK1 Antibody

SPA-07385

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Target Information
Target Name MEK1
Gene Abbr. MAP2K1
Gene ID 5604
Full Name mitogen-activated protein kinase kinase 1
Alias CFC3, MAPKK1, MEK1, MEL, MKK1
Introduction MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx. Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells. MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 61B12
Immunogen Produced by immunizing animals with full length MEK1 proteins.
Source/Purification Antibody is produced by immunizing animals with full length MEK1 proteins.
Usage
Application WB, IP
Dilutions Western Blot (1:2000); Immunoprecipitation (1:50)
MW(KDa) 45
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of total MEK1 protein. This antibody does not cross-react with MEK2 and other MAP kinase kinases.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.