MDM2 cDNA ORF Clone, Human, N-His tag - CD BioSciences

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MDM2 cDNA ORF Clone, Human, N-His tag

MDM2 cDNA ORF Clone, Human, N-His tag

SPD-10188

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Description
Full length Clone DNA of Human MDM2 oncogene, E3 ubiquitin protein ligase with N terminal His tag.
Target Information
Species Human
Target Name MDM2
Gene Abbr. MDM2
Gene ID 4193
Full Name MDM2 proto-oncogene
Alias ACTFS, HDMX, LSKB, hdm2
Introduction MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53. Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53. Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53.
Product Details
Description Full length Clone DNA of Human MDM2 oncogene, E3 ubiquitin protein ligase with N terminal His tag.
NCBI Ref Seq NM_002392.3
RefSeq ORF Size 1539 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-N-His
Promoter Enhanced CMV promoter
Tag Sequence His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
Restriction Sites KpnI + NotI (6kb + 1.54kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.