Mcl-1 Antibody - CD BioSciences

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Mcl-1 Antibody

Mcl-1 Antibody

SPA-07288

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Target Information
Target Name Mcl-1
Gene Abbr. MCL1
Gene ID 4170
Full Name MCL1 apoptosis regulator, BCL2 family member
Alias BCL2L3, EAT, MCL1-ES, MCL1L, MCL1S
Introduction Mcl-1 is an anti-apoptotic member of the Bcl-2 family originally isolated from the ML-1 human myeloid leukemia cell line during phorbol ester-induced differentiation along the monocyte/macrophage pathway. Similar to other Bcl-2 family members, Mcl-1 localizes to the mitochondria interacts with and antagonizes pro-apoptotic Bcl-2 family members and inhibits apoptosis induced by a number of cytotoxic stimuli. Mcl-1 differs from its other family members in its regulation at both the transcriptional and post-translational level. First, Mcl-1 has an extended amino-terminal PEST region, which is responsible for its relatively short half-life. Second, unlike other family members, Mcl-1 is rapidly transcribed via a PI3K/Akt dependent pathway, resulting in its increased expression during myeloid differentiation and cytokine stimulation.Mcl-1 is phosphorylated in response to treatment with phorbol ester, microtubule-damaging agents, oxidative stress, and cytokine withdrawal. Phosphorylation at Thr163, the conserved MAP kinase/ERK site located within the PEST region, slows Mcl-1 protein turnover but may prime the GSK-3 mediated phosphorylation at Ser159 that leads to Mcl-1 destabilization. Mcl-1 deficiency in mice results in peri-implantation lethality. In addition, conditional disruption of the corresponding mcl-1 gene shows that Mcl-1 plays an important role in early lymphoid development and in the maintenance of mature lymphocytes.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. SI16-04
Isotype IgG
Immunogen Recombinant Protein.
Usage
Application WB, FC, IF, IHC, IP
Dilutions Western Blot (1:1000); Flow Cytometry (1:50-1:100); Immunofluorescence (1:50-1:200); Immunohistochemistry (1:50-1:200)
MW(KDa) 40
Reactivity Human, Mouse, Rat
Storage & Handling
Storage Buffer TBS (pH 7.4), 1% BSA, 40% Glycerol.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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