LSD1 Antibody - CD BioSciences

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LSD1 Antibody

LSD1 Antibody

SPA-07046

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Target Information
Target Name LSD1
Gene Abbr. KDM1A
Gene ID 23028
Full Name lysine demethylase 1A
Alias AOF2, BHC110, CPRF, KDM1, LSD1
Introduction Lysine-specific demethylase 1 (LSD1; also known as AOF2 and BHC110) is a nuclear amine oxidase homolog that acts as a histone demethylase and transcription cofactor. Gene activation and repression is specifically regulated by the methylation state of distinct histone protein lysine residues. For example, methylation of histone H3 at Lys4 facilitates transcriptional activation by coordinating the recruitment of BPTF, a component of the NURF chromatin remodeling complex, and WDR5, a component of multiple histone methyltransferase complexes. In contrast, methylation of histone H3 at Lys9 facilitates transcriptional repression by recruiting HP1. LSD1 is a component of the CoREST transcriptional co-repressor complex that also contains CoREST, CtBP, HDAC1 and HDAC2. As part of this complex, LSD1 demethylates mono-methyl and di-methyl histone H3 at Lys4 through a FAD-dependent oxidation reaction to facilitate neuronal-specific gene repression in non-neuronal cells. In contrast, LSD1 associates with androgen receptor in human prostate cells to demethylate mono-methyl and di-methyl histone H3 at Lys9 and facilitate androgen receptor-dependent transcriptional activation. Therefore, depending on gene context LSD1 can function as either a transcriptional co-repressor or co-activator. LSD1 activity is inhibited by the amine oxidase inhibitors pargyline, deprenyl, clorgyline and tranylcypromine.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein.
Source/Purification Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino-terminus of human LSD1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP, IF, IHC, ChIP, FC
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 110
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of total LSD1 protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.