Online Inquiry
LDHA/LDHC Antibody
SPA-06872
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Lactate Dehydrogenase |
| Gene Abbr. | LDHA |
| Gene ID | 3939 |
| Full Name | lactate dehydrogenase A |
| Alias | GSD11, HEL-S-133P, LDHM, PIG19 |
| Introduction | Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites. LDHA expression is induced under hypoxic conditions. During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.While LDHA is found primarily in muscle and kidney, LDHC is a testis-specific isoform. Both proteins are associated with human disease. Mutations in the corresponding LDHA gene are associated with LDHA deficiency, which is characterized by muscle stiffness following exercise and uterine stiffness during pregnancy. Abnormal LDHC expression is associated with several forms of cancer. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C28H7 |
| Immunogen | Synthetic peptide corresponding to the sequence of human LDHA. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA. |
| Usage | |
|---|---|
| Application | WB, IHC |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 37 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of total LDHA and LDHC proteins. Species cross-reactivity for IHC-P is human only. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.