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Klotho beta Antibody
SPA-06857
| Size | Price |
| 100 µg | Online Inquiry |
| 25 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Klotho |
| Gene Abbr. | KLB |
| Gene ID | 152831 |
| Full Name | klotho beta |
| Alias | BKL |
| Introduction | Klotho beta, a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue. Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex. The Klotho beta mediated interaction of human FGF19 (mouse FGF15) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | 1025C |
| Isotype | IgG |
| Immunogen | Mouse myeloma cell line, NS0-derived recombinant human Klotho beta with a C-terminal 10 His tag. Phe53-Leu997. Accession # Q86Z14. |
| Usage | |
|---|---|
| Application | FC, IF |
| Dilutions | Flow Cytometry (0.25 µg/10^6 cells); Immunofluorescence (3-25 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human Klotho beta in direct ELISAs. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | Sodium Azide |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.