KDM4A/JMJD2A Antibody - CD BioSciences

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KDM4A/JMJD2A Antibody

KDM4A/JMJD2A Antibody

SPA-06748

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Target Information
Target Name JMJD2A
Gene Abbr. KDM4A
Gene ID 9682
Full Name lysine demethylase 4A
Alias JHDM3A, JMJD2, JMJD2A, TDRD14A
Introduction The methylation state of lysine residues in histone proteins is a major determinant of the formation of active and inactive regions of the genome and is crucial for proper programming of the genome during development. Jumonji C (JmjC) domain-containing proteins represent the largest class of potential histone demethylase proteins. The JmjC domain can catalyze the demethylation of mono-, di-, and tri-methyl lysine residues via an oxidative reaction that requires iron and α-ketoglutarate. Based on homology, both humans and mice contain at least 30 such proteins, which can be divided into 7 separate families. The jumonji domain-containing protein 2 (JMJD2) family, also known as the JmjC domain-containing histone demethylation protein 3 (JHDM3) family, contains four members: JMJD2A/JHDM3A, JMJD2B/JHDM3B, JMJD2C/JHDM3C, and JMJD2D/JHDM3D. In addition to the JmjC domain, these proteins also contain JmjN, PHD, and tudor domains, the latter of which has been shown to bind to methylated histone H3 at Lys4 and Lys9, and methylated histone H4 at Lys20. JMJD2 proteins have been shown to demethylate di- and tri-methyl histone H3 at Lys9 and Lys36 and function as both activators and repressors of transcription. JMJD2A, JMJD2C, and JMJD2D function as coactivators of the androgen receptor in prostate tumor cells. In contrast, JMJD2A also associates with Rb and NCoR corepressor complexes and is necessary for transcriptional repression of target genes. JMJD2B antagonizes histone H3 Lys9 tri-methylation at pericentric heterochromatin. JMJD2C, also known as GASC1, is amplified in squamous cell carcinomas and metastatic lung carcinoma and inhibition of JMJD2C expression decreases cell proliferation. JMJD2C has also been identified as a downstream target of Oct-4 and is critical for the regulation of self-renewal in embryonic stem cells.
Product Details
Host Sheep
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen E. coli-derived recombinant human KDM4A. Lys386-Ser514. AAH02558.
Usage
Application WB, IF
Dilutions Western Blot (1 µg/mL); Immunofluorescence (5-15 µg/mL)
MW(KDa) 150
Reactivity Human, Mouse
Specificity Detects human KDM4A in direct ELISAs and Western Blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human KDM4C is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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