Online Inquiry
IQGAP1 Antibody
SPA-06516
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IQGAP1 |
| Gene Abbr. | IQGAP1 |
| Gene ID | 8826 |
| Full Name | IQ motif containing GTPase activating protein 1 |
| Alias | HUMORFA01, SAR1, p195 |
| Introduction | IQGAPs are scaffolding proteins involved in mediating cytoskeletal function. They contain multiple protein interaction domains and bind to a growing number of molecules including actin, myosin light chain, calmodulin, E-cadherin, and β-catenin. Through their GAP-related domains, they bind the small GTPases Rac1 and cdc42. IQGAPs lack GAP activity, however, and regulate small GTPases by stabilizing their GTP-bound (active) forms. Research studies have shown that the function and distribution of the IQGAP proteins widely vary. IQGAP1 is ubiquitously expressed and has been found to interact with APC and the CLIP170 complex in response to small GTPases, promoting cell polarization and migration. Additional research studies have suggested that IQGAP1 could play a part in the invasiveness of some cancers. IQGAP2, which is about 60% identical to IQGAP1, is expressed primarily in liver but lower levels have been detected in the prostate, kidney, thyroid, stomach, and testis. Research studies have shown that IQGAP2 displays tumor suppressor properties. Less is known about the function of IQGAP3, but this protein is present in the lung, brain, small intestine, and testis and is only expressed in proliferating cells suggesting a role in cell growth and division. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D6E3J |
| Immunogen | Synthetic peptide corresponding to residues surrounding Val1272 of human IQGAP1 protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val1272 of human IQGAP1 protein. |
| Usage | |
|---|---|
| Application | WB, IP, IF |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 195 |
| Reactivity | Human, Mouse, Rat, Monkey |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of total IQGAP1 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.