IL-4 Antibody - CD BioSciences

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IL-4 Antibody

IL-4 Antibody

SPA-05913

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Target Information
Target Name IL-4
Gene Abbr. IL4
Gene ID 3565
Full Name interleukin 4
Alias BCGF-1, BCGF1, BSF-1, BSF1, IL-4
Introduction Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure. Mouse IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature mouse IL-4 shares 39%, 39%, and 59% aa sequence identity with bovine, human, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities.
Product Details
Host Goat
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen E. coli-derived recombinant cotton rat IL‑4. Cys21-Phe147. AAL18820.
Usage
Application WB
Dilutions Western Blot (0.1 µg/mL); Neutralization (0.075-0.3 µg/mL)
Reactivity D. melanogaster
Specificity Detects cotton rat IL‑4 in direct ELISAs and Western Blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat IL-4 is observed, 5% cross-reactivity with recombinant mouse IL-4 is observed, and less than 1% cross-reactivity with recombinant human IL-4 and recombinant porcine IL-4 is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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