IKKγ Antibody - CD BioSciences

service-banner

IKKγ Antibody

IKKγ Antibody

SPA-05487

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name IKKγ
Gene Abbr. IKBKG
Gene ID 8517
Full Name inhibitor of nuclear factor kappa B kinase regulatory subunit gamma
Alias AMCBX1, EDAID1, FIP-3, FIP3, Fip3p
Introduction The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.Activation of the NF-κB pathway by the T-cell lymphotrophic virus Tax protein or by TNF-α treatment leads to IKKβ-dependent phosphorylation of human IKKγ, primarily at Ser376. In mice, mutation of the orthologous residue (Ser369) to alanine leads to enhanced IKKγ-mediated stimlulation of IKKβ kinase activity.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. DA10-12
Immunogen Produced by immunizing animals with full length human GST-IKKγ protein.
Source/Purification Antibody is produced by immunizing animals with full length human GST-IKKγ protein.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 48
Reactivity Human, Rat
Sensitivity Endogenous
Specificity It detects endogenous levels of total IKKγ protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.