IKKβ Antibody - CD BioSciences

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IKKβ Antibody

IKKβ Antibody

SPA-05477

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Target Information
Target Name IKKβ
Gene Abbr. IKBKB
Gene ID 3551
Full Name inhibitor of nuclear factor kappa B kinase subunit beta
Alias IKK-beta, IKK2, IKKB, IMD15, IMD15A
Introduction The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 10A9B6
Isotype IgG1 Kappa
Immunogen Full-length human IKKb/IKK2 recombinant protein was used as immunogen (NP_001547).
Usage
Application WB, IF, IP
Dilutions Western Blot (2-5 µg/mL); Flow Cytometry (1:10-1:1000); Immunoprecipitation (2-5 µg/10^6 cells)
MW(KDa) 87
Reactivity Human, Mouse
Specificity An approx. 87 kDa band should be observed.
Storage & Handling
Storage Buffer PBS.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.