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IGFBP-3 Antibody
SPA-05435
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | IGFBP-3 |
| Gene Abbr. | IGFBP3 |
| Gene ID | 3486 |
| Full Name | insulin like growth factor binding protein 3 |
| Alias | BP-53, IBP3 |
| Introduction | The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. Mouse IGFBP-3 cDNA encodes a 291 amino acid (aa) residue precursor protein with a putative 27 aa residue signal peptide that is processed to generate the 264 aa residue mature protein with three potential N-linked glycosylation sites. Mouse and human IGFBP-3 share 81% amino acid homology. Mouse IGFBP-3 is expressed in multiple tissues. Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57. |
| Product Details | |
|---|---|
| Host | Rat |
| Clonality | Monoclonal |
| Clone No. | 138202 |
| Isotype | IgG2B |
| Immunogen | Mouse myeloma cell line NS0-derived recombinant mouse IGFBP‑3. Pro22-Gln291 (Arg250Gln, Gln259Arg, and Arg271Pro). Accession # P47878. |
| Usage | |
|---|---|
| Application | WB, ELISA |
| Dilutions | Western Blot (1 µg/mL); ELISA Capture (2-8 µg/mL); ELISA Detection (0.1-0.4 µg/mL) |
| Reactivity | Mouse |
| Specificity | Detects mouse IGFBP-3 in direct ELISAs and Western Blots. In Western Blots, this antibody does not cross-react with recombinant human IGFBP‑1, rhIGFBP‑2, rhIGFBP‑3, rhIGFBP‑4, rhIGFBP‑5, rhIGFBP‑6, recombinant mouse (rm) IGFBP‑2, rmIGFBP‑5, or rmIGFBP‑6. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.