HP1γ Antibody - CD BioSciences

service-banner

HP1γ Antibody

HP1γ Antibody

SPA-05234

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name HP1γ
Gene Abbr. CBX3
Gene ID 11335
Full Name chromobox 3
Alias HECH, HP1-GAMMA, HP1Hs-gamma
Introduction Heterochromatin protein 1 (HP1) is a family of heterochromatic adaptor molecules involved in both gene silencing and higher order chromatin structure. All three HP1 family members (α, β, and γ) are primarily associated with centromeric heterochromatin; however, HP1β and γ also localize to euchromatic sites in the genome. HP1 proteins are approximately 25 kDa in size and contain a conserved amino-terminal chromodomain, followed by a variable hinge region and a conserved carboxy-terminal chromoshadow domain. The chromodomain facilitates binding to histone H3 tri-methylated at Lys9, a histone "mark" closely associated with centromeric heterochromatin. The variable hinge region binds both RNA and DNA in a sequence-independent manner. The chromoshadow domain mediates the dimerization of HP1 proteins, in addition to binding multiple proteins implicated in gene silencing and heterochromatin formation, including the SUV39H histone methyltransferase, the DNMT1 and DNMT3a DNA methyltransferases, and the p150 subunit of chromatin-assembly factor-1 (CAF1). In addition to contributing to heterochromatin formation and propagation, HP1 and SUV39H are also found complexed with retinoblastoma (Rb) and E2F6 proteins, both of which function to repress euchromatic gene transcription in quiescent cells. HP1 proteins are subject to multiple types of post-translational modifications, including phosphorylation, acetylation, methylation, ubiquitination, and sumoylation, suggesting multiple means of regulation.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic peptide corresponding to the amino terminus of human HP1gamma.
Source/Purification Antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human HP1gamma. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP, IF, FC
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 22
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of total HP1gamma protein. The antibody does not cross-react HP1alpha or HP1beta proteins.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.