Histone H3 Antibody - CD BioSciences

service-banner

Histone H3 Antibody

Histone H3 Antibody

SPA-05154

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name Histone H3
Gene Abbr. H3C14
Gene ID 126961
Full Name H3 clustered histone 14
Alias H3, H3.2, H3/M, H3C13, H3C15
Introduction The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype Serum
Immunogen Histone H3 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to the C-terminus region of human Histone H3.
Usage
Application WB, ELISA, IHC
Dilutions Western Blot (1:2000); ELISA (1:10000); Immunohistochemistry (1:100)
MW(KDa) 17
Reactivity Human
Storage & Handling
Storage Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.
Preservative 0.01% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.