Histone H3 Antibody - CD BioSciences

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Histone H3 Antibody

Histone H3 Antibody

SPA-05121

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Target Information
Target Name Histone H3
Gene Abbr. H3-3A
Gene ID 3020
Full Name H3.3 histone A
Alias H3-3B, H3.3A, H3F3, H3F3A
Introduction Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 1B1B2
Immunogen peptide specific to the carboxy terminus of human histone H3 protein.
Source/Purification Antibody is produced by immunizing animals with a peptide specific to the carboxy terminus of human histone H3 protein.
Usage
Application WB, IP, IF, IHC, ChIP, FC
Dilutions Western Blot (1:1000)
MW(KDa) 17
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It recognizes endogenous levels of total histone H3 protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.