GADD153/CHOP Antibody - CD BioSciences

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GADD153/CHOP Antibody

GADD153/CHOP Antibody

SPA-02205

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Target Information
Target Name CHOP
Gene Abbr. DDIT3
Gene ID 1649
Full Name DNA damage inducible transcript 3
Alias AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10
Introduction CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner. CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase. Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death. Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum. The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen A synthetic peptide made to an internal portion of the human CHOP/GADD153 protein (between residues 100-150) [UniProt P35638].
Usage
Application WB, FC, IHC
Dilutions Western Blot (1:1000); Flow Cytometry (2-5 μg/10^6 cells); Immunohistochemistry (1:400)
MW(KDa) 27
Reactivity Human, Mouse, Rat, Primate
Storage & Handling
Storage Buffer PBS (pH 7.4).
Preservative 0.02% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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