GADD153/CHOP Antibody - CD BioSciences

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GADD153/CHOP Antibody

GADD153/CHOP Antibody

SPA-02202

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Target Information
Target Name CHOP
Gene Abbr. DDIT3
Gene ID 1649
Full Name DNA damage inducible transcript 3
Alias AltDDIT3, C/EBPzeta, CEBPZ, CHOP, CHOP-10
Introduction CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner. CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase. Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death. Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum. The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D46F1
Immunogen Synthetic peptide corresponding to residues surrounding Leu159 of human CHOP protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu159 of human CHOP protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 27
Reactivity Mouse
Sensitivity Endogenous
Specificity It detects endogenous levels of total CHOP protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.