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FKBP1A/FKBP12 Antibody
SPA-04004
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | FKBP12 |
| Gene Abbr. | FKBP1A |
| Gene ID | 2280 |
| Full Name | FKBP prolyl isomerase 1A |
| Alias | FKBP-12, FKBP-1A, FKBP1, FKBP12, PKC12 |
| Introduction | FKBP12 (FKBP1A) is a prototypical member of the FKBP (FK506 binding protein) family of immunophilins, so named because of the ability of FKBP12 to bind to the immunosuppressive drug FK506 (tacrolimus). The protein is the smallest member in the family and contains only one peptidylprolyl isomerase (PPIase) core domain (FK domain) responsible for its PPIase activity, and its binding to FK506 and other compounds (e.g., rapamycin). When bound to FK506 or rapamycin, the protein:drug complex further binds and inhibits two important signaling molecules: calcineurin, a key enzyme in T cell activation; and the metabolic enzyme mTOR. The inhibition of these pathways has been functionally linked to immunosuppression. Through its binding properties, FKBP12 also plays regulatory roles in other pathways. For example, the ryanodine receptor (RyR), a type of Ca2+ release channel, exhibits leakiness in the absence of FKBP12 binding, leading to reduced muscle contractility. FKBP12 can also bind TGFBR1 and prevent ligand independent activation. The protein also mediates MDM2 degradation by binding and disrupting MDM2/MDM4 association, thereby inducing MDM2 self-ubiquitination and enhancing the sensitivity of cells to chemotherapy induced cellular apoptosis. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Phe49 of human FKBP1A/FKBP12 protein. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe49 of human FKBP1A/FKBP12 protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 12 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of total FKBP1A/FKBP12 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.