Di/Tri-Methyl-Histone H3 (Lys9) Antibody - CD BioSciences

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Di/Tri-Methyl-Histone H3 (Lys9) Antibody

Di/Tri-Methyl-Histone H3 (Lys9) Antibody

SPA-05119

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Target Information
Target Name Histone H3
Gene Abbr. H3-3A
Gene ID 3020
Full Name H3.3 histone A
Alias H3-3B, H3.3A, H3F3, H3F3A
Introduction The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. Histone methylation is a major determinant for the formation of active and inactive regions of the genome and is crucial for the proper programming of the genome during development. Arginine methylation of histones H3 (Arg2, 17, 26) and H4 (Arg3) promotes transcriptional activation and is mediated by a family of protein arginine methyltransferases (PRMTs), including the co-activators PRMT1 and CARM1 (PRMT4). In contrast, a more diverse set of histone lysine methyltransferases has been identified, all but one of which contain a conserved catalytic SET domain originally identified in the Drosophila Su(var)3-9, Enhancer of zeste, and Trithorax proteins. Lysine methylation occurs primarily on histones H3 (Lys4, 9, 27, 36, 79) and H4 (Lys20) and has been implicated in both transcriptional activation and silencing. Methylation of these lysine residues coordinates the recruitment of chromatin modifying enzymes containing methyl-lysine binding modules such as chromodomains (HP1, PRC1), PHD fingers (BPTF, ING2), tudor domains (53BP1), and WD-40 domains (WDR5). The discovery of histone demethylases such as PADI4, LSD1, JMJD1, JMJD2, and JHDM1 has shown that methylation is a reversible epigenetic marker.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 6F12
Immunogen Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is tri-methylated.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is tri-methylated.
Usage
Application WB, IP, IF, ChIP
Dilutions Western Blot (1:2000); Immunoprecipitation (1:100)
MW(KDa) 17
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of histone H3 when di- or tri-methylated on Lys9. The antibody also shows slight cross-reactivity with histone H3 when mono-methylated on Lys9. The antibody does not cross-react with methylated histone H3 Lys4, Lys27, Lys36 or Lys79.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.