DDB-2 Antibody - CD BioSciences

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DDB-2 Antibody

DDB-2 Antibody

SPA-02849

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Target Information
Target Name DDB-2
Gene Abbr. DDB2
Gene ID 1643
Full Name damage specific DNA binding protein 2
Alias DDBB, UV-DDB2, XPE
Introduction Damaged DNA-Binding Protein (DDB) consists of a 127 kDa subunit (DDB-1) and a 48 kDa subunit (DDB-2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB). In conjunction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway. Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients. DDB-1 is a relatively abundant protein that is vital for normal cell function and is evolutionarily conserved in mammals, insects, worms and plants. Unlike DDB-2, lesions in DDB-1 have yet to be indentified in XP-E patients. In association with ROC-1 and CUL4A, DDB-1 functions to recruit substrate-specific targeting subunits, generally known as DCAFs or CDWs, to CUL4-RING E3 ubiquitin-protein ligase complexes. Ubiquitination of histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage by the DDB1-DDB2-CUL4A-ROC1 E3 ubiquitin-protein ligase complex may facilitate their removal from the nucleosome in order to promote DNA repair. DDB-1, in association with other CUL4-based E3 ligase complexes, has also been found to be a regulator of mTOR signaling.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D4C4
Isotype IgG
Immunogen Synthetic peptide corresponding to amino acids surrounding Ala174 of human DDB-2.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ala174 of human DDB-2.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 43
Reactivity Human, Mouse
Sensitivity Endogenous
Specificity Recognizes endogenous levels of total DDB-2 protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.