DDB-1 Antibody - CD BioSciences

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DDB-1 Antibody

DDB-1 Antibody

SPA-02842

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Target Information
Target Name DDB-1
Gene Abbr. DDB1
Gene ID 1642
Full Name damage specific DNA binding protein 1
Alias DDBA, UV-DDB1, XAP1, XPCE, XPE
Introduction Damaged DNA-Binding Protein (DDB) consists of a 127 kDa subunit (DDB-1) and a 48 kDa subunit (DDB-2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB). In conjunction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway. Loss of DDB activity has been identified in a subset of xeroderma pigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients. DDB-1 is a relatively abundant protein that is vital for normal cell function and is evolutionarily conserved in mammals, insects, worms and plants. Unlike DDB-2, lesions in DDB-1 have yet to be indentified in XP-E patients. In association with ROC-1 and CUL4A, DDB-1 functions to recruit substrate-specific targeting subunits, generally known as DCAFs or CDWs, to CUL4-RING E3 ubiquitin-protein ligase complexes. Ubiquitination of histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage by the DDB1-DDB2-CUL4A-ROC1 E3 ubiquitin-protein ligase complex may facilitate their removal from the nucleosome in order to promote DNA repair. DDB-1, in association with other CUL4-based E3 ligase complexes, has also been found to be a regulator of mTOR signaling.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Recombinant protein encompassing a sequence within the C-terminus region of human DDB1.
Usage
Application WB, IF, IHC, IP
Dilutions Western Blot (1:500-1:10000); Immunofluorescence (1:100-1:1000); Immunohistochemistry (1:100-1:1000); Immunoprecipitation (1:500-1:1000)
MW(KDa) 127
Reactivity Human, Mouse, Rat, Bovine, Chicken, Xenopus
Storage & Handling
Storage Buffer 1X PBS, 1% BSA, 20% Glycerol (pH 7).
Preservative 0.025% Proclin 300
Storage Temp. Aliquot and store at -20 °C or -80 °C.
Handling Avoid freeze-thaw cycles.

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