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DARC Antibody
SPA-02800
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | DARC |
| Gene Abbr. | ACKR1 |
| Gene ID | 2532 |
| Full Name | atypical chemokine receptor 1 (Duffy blood group) |
| Alias | CCBP1, CD234, DARC, DARC/ACKR1, Dfy |
| Introduction | DARC, also known as the Duffy antigen/chemokine receptor, is a seven-transmembrane protein homologous to the classical chemokine G-protein coupled receptors (GPCRs) with the exception of the motif required for G protein coupling. DARC can bind with high affinity several chemokines without transducing any signal, suggesting it may modulate the signals normally induced by these chemokines. Recently, DARC was found to interact with KAI1, a four transmembrane protein recently identified as a tumor metastasis suppressor protein. It is thought that tumor cells dislodged from the primary tumor and expressing KAI1 interact with DARC proteins expressed on vascular cells, transmitting a senescent signal to the tumor cells, while tumor cells that have lost KAI1 expression can proliferate and potentially give rise to metastases. At least three isoforms of DARC are known to exist. |
| Product Details | |
|---|---|
| Host | Sheep |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant mouse DARC. Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285. NP_034175. |
| Usage | |
|---|---|
| Application | WB, FC, IHC |
| Dilutions | Western Blot (1 µg/mL); Flow Cytometry (0.25 µg/10^6 cells); Immunohistochemistry (5-15 µg/mL) |
| Reactivity | Mouse, Rat |
| Specificity | Detects mouse and rat DARC in Western Blots. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.