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CXCL4/PF4 Antibody
SPA-02515
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | CXCL4/PF4 |
| Gene Abbr. | PF4 |
| Gene ID | 5196 |
| Full Name | platelet factor 4 |
| Alias | CXCL4, PF-4, SCYB4 |
| Introduction | CXCL4, also known as Platelet Factor 4, is a member of the CXC chemokine family, CXCL4. Human CXCL4 is a 101 amino acid (aa) protein with a 32 aa signal sequence and a 70 aa mature protein. CXCL4 has homology with IL-8 and beta -thromboglobulin. Human and mouse CXCL4 share a 64% identity. CXCL4 contains several heparin-binding sites at the C‑terminal region. The active protein consists of a tetramer composed of individual CXCL4 subunits. Megakaryocytes synthesize CXCL4 and store it as tetramers in alpha -granules. The CXCL4 tetramers are secreted by activated platelets and can be measured at micromolar levels in serum. In contrast to other CXC chemokines, CXCL4 lacks chemotactic activity for polymorphonuclear granulocytes. CXCL4 does not contain an ELR motif. However, many other functions have been observed for CXCL4. CXCL4 is involved in monocyte survivial and differentiation into macrophages, and it has anti-angiogenic activity. CXCL4 has been demonstrated to inhibit the binding of FGF-2 to high-affinity receptors and its subsequent internalization. Cell surface neutrophil chondroitin sulfate chains serve as CXCL4 binding sites; affinity is controlled by the degree of sulfation of these chains. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 170131 |
| Isotype | IgG2B |
| Immunogen | E. coli-derived recombinant human CXCL4/PF4. Glu32-Ser101. P02776.2. |
| Usage | |
|---|---|
| Application | ELISA |
| Dilutions | ELISA Capture (2-8 µg/mL); ELISA Detection (0.1-0.4 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human CXCL4/PF4 in ELISAs. In ELISAs, this antibody does not cross-react with recombinant human (rh) CXCL1, rhCXCL2, or rmCXCL4. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.