Cdc42 Antibody - CD BioSciences

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Cdc42 Antibody

Cdc42 Antibody

SPA-02110

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
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Target Information
Target Name Cdc42
Gene Abbr. CDC42
Gene ID 998
Full Name cell division cycle 42
Alias CDC42Hs, G25K, TKS
Introduction Rac and Cdc42 are members of the Rho-GTPase family. In mammals, Rac exists as three isoforms, Rac1, Rac2 and Rac3, which are highly similar in sequence. Rac1 and Cdc42, the most widely studied of this group, are ubiquitously expressed. Rac2 is expressed in cells of hematopoietic origin, and Rac3, while highly expressed in brain, is also found in many other tissues. Rac and Cdc42 play key signaling roles in cytoskeletal reorganization, membrane trafficking, transcriptional regulation, cell growth and development. GTP binding stimulates the activity of Rac/Cdc42, and the hydrolysis of GTP to GDP through the protein's intrinsic GTPase activity, rendering it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). Another level of regulation is achieved through the binding of RhoGDI, a guanine nucleotide dissociation inhibitor, which retains Rho family GTPases, including Rac and Cdc42, in their inactive GDP-bound state.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic peptide corresponding to residues surrounding Lys135 of human Cdc42.
Source/Purification Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys135 of human Cdc42. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 21
Reactivity Human, Mouse, Rat, Monkey, Bovine
Sensitivity Endogenous
Specificity It detects endogenous levels of total Cdc42 protein. The antibody does not cross-react with other small GTPases.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.