Caspase-9 Sandwich ELISA Kit - CD BioSciences

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Caspase-9 Sandwich ELISA Kit

Caspase-9 Sandwich ELISA Kit

SPK-00150

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1 X 96T Online Inquiry
5 X 96T Online Inquiry
10 X 96T Online Inquiry
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Target Information
Target Name Caspase-9
Gene Abbr. CASP9
Gene ID 842
Full Name caspase 9
Alias APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56
Introduction Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330 producing a p37 subunit that can serve to amplify the apoptotic response. Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Assay Principle quantitative
Sensitivity 0.195 ng/mL
Detection Range 0.78 ng/mL - 50 ng/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Precision Intra-assay Precision (Precision within an assay): CV%<8%.
Inter-assay Precision (Precision between assays): CV%<10%.
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

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