c-Myc Antibody - CD BioSciences

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c-Myc Antibody

c-Myc Antibody

SPA-01234

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25 µg Online Inquiry
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Target Information
Target Name c-Myc
Gene Abbr. MYC
Gene ID 1386
Full Name activating transcription factor 2
Alias MRTL, MYCC, bHLHe39, c-Myc
Introduction The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2. In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2.ATF-7 is another member of the ATF/CREB family of leucine zipper proteins. Similarly, Thr51 and Thr53 (corresponding to Thr69 and Thr71 of ATF-2, respectively) can be phosphorylated under different conditions.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. 2270A
Isotype IgG
Immunogen E.coli-derived recombinant human c-Myc. Arg66-Asp201. P01106.
Usage
Application WB, FC, IF
Dilutions Western Blot (1 µg/mL); Immunofluorescence (3-25 µg/mL); Flow Cytometry (0.25 µg/10^6 cells)
MW(KDa) 62
Reactivity Human
Validation Knockout Validated.
Specificity Detects human c-Myc in direct ELISAs.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.