Online Inquiry
BiP Antibody
SPA-01073
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | BiP |
| Gene Abbr. | HSPA5 |
| Gene ID | 3309 |
| Full Name | heat shock protein family A (Hsp70) member 5 |
| Alias | BIP, GRP78, HEL-S-89n |
| Introduction | Secretory and transmembrane proteins are synthesized on polysomes and translocated into the endoplasmic reticulum (ER). Inside the ER, these proteins are often modified by disulfide bond formation, amino-linked glycosylation and folding. To help proteins fold properly, the ER contains a pool of molecular chaperones including BiP. BiP was identified as an immunoglobulin heavy chain binding protein in pre-B cells. It was also found to be induced at the protein level by glucose starvation. When protein folding is disturbed inside ER, BiP synthesis is increased. Subsequently, BiP binds to misfolded proteins to prevent them from forming aggregates and assists in proper refolding. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | C50B12 |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to residues surrounding Gly584 of human BiP. |
| Source/Purification | Antibody is produced by immunizing rabbits with a synthetic peptide corresponding to residues surrounding Gly584 of human BiP. |
| Usage | |
|---|---|
| Application | WB, IHC, FC |
| Dilutions | Western Blot (1:1000); Immunohistochemistry (1:200); Flow Cytometry (1:200) |
| MW(KDa) | 78 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | Detects endogenous levels of total BiP protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.