Bim Antibody - CD BioSciences

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Bim Antibody

Bim Antibody

SPA-01067

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100 µg Online Inquiry
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Target Information
Target Name BIM
Gene Abbr. BCL2L11
Gene ID 10018
Full Name BCL2 like 11
Alias BAM, BIM, BOD
Introduction Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains. Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1. Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated. Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS. The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis. Apoptotic activity of these longer isoforms may be regulated by phosphorylation. Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.
Product Details
Host Goat
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Peptide with sequence C-FNAYYARRLEK corresponding to C-Terminus according to NP_619528.1, NP_619529.1, NP_996886.1.
Usage
Application WB, ELISA
Dilutions Western Blot (0.5-2 µg/mL); ELISA (1:16000)
MW(KDa) 12, 15, 23
Reactivity Human
Specificity This antibody is expected to recognise at least reported isoforms Bim-alpha1 (BimABCD), Bim-alpha2 (BimACD) and Bim-alpha3 (BimAD).
Storage & Handling
Storage Buffer Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA.
Preservative 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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