Bim Antibody - CD BioSciences

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Bim Antibody

Bim Antibody

SPA-01065

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Target Information
Target Name BIM
Gene Abbr. BCL2L11
Gene ID 10018
Full Name BCL2 like 11
Alias BAM, BIM, BOD
Introduction Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains. Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1. Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated. Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS. The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis. Apoptotic activity of these longer isoforms may be regulated by phosphorylation. Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen A peptide corresponding to amino acids near the center of human Bim. The sequence is identical to that of mouse and rat. The immunogen is located within amino acids 80 - 130 of Bim. Amino Acid Squence: SGYFSFDTDRSPAPMS.
Usage
Application WB, ELISA, IF
Dilutions Western Blot (5 µg/mL); ELISA (1:100-1:2000); Immunofluorescence (10 µg/mL); Immunofluorescence (20 µg/mL)
MW(KDa) 12, 15, 23
Reactivity Human, Mouse, Rat
Specificity Human BIM has 3 isoforms, including isoform EL (198aa, 22kD), isoform L (138aa, 16kD) and isoform S (108aa, 13kD). Mouse BIM has 3 isoforms, including isoform EL (196aa, 22kD), isoform L (140aa, 16kD) and isoform S (110aa, 13kD). Rat BIM has 4 isoforms, including isoform BOD-L (196aa, 22kD), isoform L (140aa, 16kD) and isoform BOD-M (110aa, 13kD). It only detects isoform EL.
Storage & Handling
Storage Buffer PBS.
Preservative 0.02% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.