Bim Antibody - CD BioSciences

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Bim Antibody

Bim Antibody

SPA-01064

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100 µg Online Inquiry
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Target Information
Target Name BIM
Gene Abbr. BCL2L11
Gene ID 10018
Full Name BCL2 like 11
Alias BAM, BIM, BOD
Introduction Bim/Bod is a pro-apoptotic protein belonging to the BH3-only group of Bcl-2 family members including Bad, Bid, Bik, Hrk, and Noxa that contain a BH3 domain but lack other conserved BH1 or BH2 domains. Bim induces apoptosis by binding to and antagonizing anti-apoptotic members of the Bcl-2 family. Interactions have been observed with Bcl-2, Bcl-xL, Mcl-1, Bcl-w, Bfl-1, and BHRF-1. Bim functions in regulating apoptosis associated with thymocyte negative selection and following growth factor withdrawal, during which Bim expression is elevated. Three major isoforms of Bim are generated by alternative splicing: BimEL, BimL, and BimS. The shortest form, BimS, is the most cytotoxic and is generally only transiently expressed during apoptosis. The BimEL and BimL isoforms may be sequestered to the dynein motor complex through an interaction with the dynein light chain and released from this complex during apoptosis. Apoptotic activity of these longer isoforms may be regulated by phosphorylation. Environmental stress triggers Bim phosphorylation by JNK and results in its dissociation from the dynein complex and increased apoptotic activity.
Product Details
Host Rat
Clonality Monoclonal
Clone No. 3C5
Isotype IgG2A Kappa
Immunogen Synthetic peptide corresponding to aa 20-40 of mouse Bim.
Usage
Application WB, ELISA, FC, IF, IHC, IP
Dilutions Western Blot (1-5 µg/mL); Immunoprecipitation (1-2 µg/mL)
MW(KDa) 12, 15, 23
Reactivity Human, Mouse, Rat, Canine, Primate
Specificity Recognizes an epitope within aa 20-40 of human, mouse, rat, monkey and dog BimL (~19kDa), BimEL (~23kDa), and BimS (~16kDa). Also recognizes the six novel isoforms of human Bim, generated by alternative splicing and lacking the C-terminal hydrophobic region.
Storage & Handling
Storage Buffer PBS containing 25% glycerol.
Preservative 0.02% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.