Beclin-1 Antibody - CD BioSciences

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Beclin-1 Antibody

Beclin-1 Antibody

SPA-01019

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Target Information
Target Name Beclin-1
Gene Abbr. BECN1
Gene ID 8678
Full Name beclin 1
Alias ATG6, VPS30, beclin1
Introduction Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions. One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6/Vps30. Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells. Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak. While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers. Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export. Investigators have demonstrated that Beclin-1-/- mice die early in embryogenesis and Beclin-1-/+ mice have a high incidence of spontaneous tumors. Stem cells from the null mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal. Researchers have also found that overexpression of Beclin-1 in virally infected neurons in vivo resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis.To induce autophagy, AMP-activated protein kinase (AMPK) directly phosphorylates Beclin-1 at conserved Ser93 and Ser96 residues in human, which correspond to murine Ser91 and Ser94.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 2A4
Isotype IgG1
Immunogen Purified recombinant fragment of human BECN1 expressed in E. coli.
Usage
Application WB, ELISA, FC, IHC
Dilutions Western Blot (1:500-1:2000); ELISA (1:10000); Flow Cytometry (1:200-1:400); Immunohistochemistry (1:10-1:500)
MW(KDa) 60
Reactivity Human
Storage & Handling
Storage Buffer Ascites.
Preservative 0.03% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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