Bcr Antibody - CD BioSciences

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Bcr Antibody

Bcr Antibody

SPA-01004

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Target Information
Target Name BCR
Gene Abbr. BCR
Gene ID 613
Full Name BCR activator of RhoGEF and GTPase
Alias ALL, BCR1, CML, D22S11, D22S662
Introduction The Bcr gene was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The amino-terminal region of Bcr contains an oligomerization domain, a serine/threonine kinase domain, and a region that binds SH2 domains. The middle of the protein has a PH domain and a region of sequence similarity to the guanine nucleotide exchange factors for the Rho family of GTP binding proteins. The carboxy-terminal region may be involved in a GTPase activating function for the small GTP-binding protein Rac. The function of wild type Bcr in cells remains unclear. PDGF receptor may use Bcr as a downstream signaling mediator. Research studies have shown that the Bcr-Abl fusion results in production of a constitutively active tyrosine kinase, which causes chronic myelogenous leukemia (CML). Tyr177 of Bcr is phosphorylated in the Bcr-Abl fusion protein, which plays an important role in transforming the activity of Bcr-Abl. Phosphorylated Tyr177 provides a docking site for Gab2 and GRB2.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen The immunogen recognized by this antibody maps to a region between residue 1221 and 1271 of human breakpoint cluster region using the numbering given in entry NP_004318.3 (GeneID 613).
Usage
Application WB, FC, IF, IHC, IP
Dilutions Western Blot (1:2000-1:10000); Flow Cytometry (0.5 μg/10^6 cells in 150 µL); Immunofluorescence (1:500-1:2000); Immunohistochemistry (1:500-1:2000); Immunoprecipitation (10 μg/mg lysate)
MW(KDa) 130, 160 (Bcr); 210 (Bcr-Abl)
Reactivity Human
Storage & Handling
Storage Buffer Tris-Citrate/Phosphate (pH 7.0-8.0).
Preservative 0.09% Sodium Azide
Storage Temp. Store at 4 °C.
Handling Do not freeze.

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