Bcl-2 Antibody - CD BioSciences

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Bcl-2 Antibody

Bcl-2 Antibody

SPA-00939

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
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Target Information
Target Name Bcl-2
Gene Abbr. BCL2
Gene ID 596
Full Name BCL2 apoptosis regulator
Alias Bcl-2, PPP1R50
Introduction Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release. It has been implicated in modulating mitochondrial calcium homeostasis and proton flux. Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74, and Ser87. It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events. Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes. Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 100/D5
Isotype IgG1 Kappa
Immunogen The synthetic peptide corresponding to amino acids range from 41-64 (GAAPAPGIFSSQPG-Cys), of human Bcl-2 was used as immunogen to generate the antibody; GenBank no. NP_000648.2 (Pezzella et al, 1990).
Usage
Application WB, ELISA, IF, IHC, IP
Dilutions Western Blot (0.5-1 µg/mL); ELISA (1-5 µg/mL); Flow Cytometry (0.5-1 µg/10^6 cells); Immunofluorescence (1-2 µg/mL); Immunoprecipitation (1-2 µg/500 µg protein lysate)
MW(KDa) 26
Reactivity Human, Mouse, Rat
Specificity This antibody recognizes a protein of 25-26kDa, identified as the bcl-2alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression of bcl-2alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.
Storage & Handling
Storage Buffer 10 mM PBS with 0.05% BSA.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C.

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