Bcl-10 Antibody - CD BioSciences

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Bcl-10 Antibody

Bcl-10 Antibody

SPA-00928

Size Price
0.1 mL Online Inquiry
0.025 mL Online Inquiry
More Options Online Inquiry
Target Information
Target Name Bcl-10
Gene Abbr. BCL10
Gene ID 8915
Full Name BCL10 immune signaling adaptor
Alias CARMEN, CIPER, CLAP, IMD37, c-E10
Introduction Bcl10/CIPER/CLAP/mE10 is a widely expressed CARD (caspase recruitment domain) containing protein shown to induce apoptosis and activate NF-κB. The CARD domain mediates self-oligomerization, interactions with other CARD proteins and is necessary for NF-κB activation, although the precise mechanism which Bcl10 regulates these processes is not fully understood. The discovery of Bcl10 came from observations of the chromosomal translocation t(1;14)(p22;q32) from B cell lymphomas of the mucosa-associated lymphoid tissue (MALT). This translocation results in deregulated expression of a truncated form of Bcl10 which lacks apoptotic activity and enhances transformation. Studies from Bcl10 deficient mice demonstrate that Bcl10 is essential for the activation of NF-κB by T- and B-cell receptors. One third of Bcl10 deficient mice developed lethal exencephaly. Surviving mice were unaffected by various apoptotic stimuli, but were severely immunodeficient and defective in antigen receptor-induced NF-κB activiation. PKC or T-cell receptor signaling results in a downregulation of Bcl10 protein levels, attenuating both NF-κB activation and cellular proliferation and also provides a negative feedback regulation of the NF-κB signaling to T cell signaling.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. SPM520
Isotype IgG1 Kappa
Immunogen Human BCL10 recombinant protein (epitope aa122-168).
Usage
Application WB, FC, IF, IHC, IP
Dilutions Western Blot (0.5-1 µg/mL); Flow Cytometry (0.5-1 µg/10^6 cells); Immunofluorescence (1-2 µg/mL); Immunoprecipitation (1-2 µg/500 µg)
MW(KDa) 28
Reactivity Human
Specificity This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas.
Storage & Handling
Storage Buffer 10 mM PBS with 0.05% BSA.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C.

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