Bax Antibody - CD BioSciences

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Bax Antibody

Bax Antibody

SPA-00907

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name BAX
Gene Abbr. BAX
Gene ID 581
Full Name BCL2 associated X, apoptosis regulator
Alias BCL2L4
Introduction Bax is a key component for cellular induced apoptosis through mitochondrial stress. Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane. Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 2D2
Isotype IgG1 Kappa
Immunogen A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Usage
Application WB, FC, IF, IHC
Dilutions Western Blot (0.5-1 µg/mL); Flow Cytometry (0.5-1 µg/10^6 cells); Immunofluorescence (1-2 µg/mL); Immunohistochemistry (0.5-1 µg/mL)
MW(KDa) 20
Reactivity Human, Primate, Mouse, Rat
Specificity Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Storage & Handling
Storage Buffer 10 mM PBS with 0.05% BSA.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.