Bad Sandwich ELISA Kit - CD BioSciences

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Bad Sandwich ELISA Kit

Bad Sandwich ELISA Kit

SPK-00084

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1 X 96T Online Inquiry
5 X 96T Online Inquiry
10 X 96T Online Inquiry
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Target Information
Target Name BAD
Gene Abbr. Bad
Gene ID 12015
Full Name BCL2-associated agonist of cell death
Alias AI325008, Bbc2
Introduction Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.
Product Details
Measurement Sandwich
Usage
Application ELISA
Reactivity
Assay Principle quantitative
Sensitivity 7.8 pg/mL
Detection Range 31.2 pg/mL - 2000 pg/mL
Sample Volume 50-100μL
Assay Time 1-5h
Detection Wavelength 450 nm
Precision Intra-assay Precision (Precision within an assay): CV%<8%.
Inter-assay Precision (Precision between assays): CV%<10%.
Storage & Handling
Storage Temp. Store at 2-8°C. Please refer to protocol.

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