Bad Antibody - CD BioSciences

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Bad Antibody

Bad Antibody

SPA-00834

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100 µg Online Inquiry
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Target Information
Target Name BAD
Gene Abbr. BAD
Gene ID 572
Full Name BCL2 associated agonist of cell death
Alias BBC2, BCL2L8
Introduction Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen KLH-coupled human Bad synthetic peptide. MFQIPEFEPSEQEDSSSAERGC. Q92934.
Usage
Application WB, IF
Dilutions Western Blot (1 µg/mL); Immunofluorescence (0.3-15 µg/mL)
MW(KDa) 21-23
Reactivity Human, Mouse
Specificity Detects human and mouse Bad in Western Blots.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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