Atg5 Antibody - CD BioSciences

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Atg5 Antibody

Atg5 Antibody

SPA-00711

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0.025 mg Online Inquiry
0.1 mg Online Inquiry
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Target Information
Target Name Atg5
Gene Abbr. ATG5
Gene ID 9474
Full Name autophagy related 5
Alias APG5, APG5-LIKE, APG5L, ASP, SCAR25
Introduction Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. The molecular machinery of autophagy was largely discovered in yeast and referred to as autophagy-related (Atg) genes. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D5G3
Immunogen Synthetic peptide corresponding to residues surrounding Gly232 of human Atg5 protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly232 of human Atg5 protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 55
Reactivity Human, Monkey
Sensitivity Endogenous
Specificity It recognizes endogenous levels of total Atg5 protein. This antibody is capable of detecting Atg5 when conjugated to Atg12.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.