Atg4B Antibody - CD BioSciences

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Atg4B Antibody

Atg4B Antibody

SPA-00689

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0.1 mg Online Inquiry
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Target Information
Target Name Atg4B
Gene Abbr. ATG4B
Gene ID 23192
Full Name autophagy related 4B cysteine peptidase
Alias APG4B, AUTL1
Introduction Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog.Phosphorylation of Atg4B by MST4 increases its activity and autophagic flux.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen A portion of amino acid 350-400 of human APG4B was used as the immunogen.
Usage
Application WB, IHC
Dilutions Western Blot (1-3 µg/mL)
MW(KDa) 48
Reactivity Human, Mouse, Rat, Canine, Primate, Primate-Orangutan
Storage & Handling
Storage Buffer 0.2 mL PBS and 0.2% Gelatin.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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