Atg4A Antibody - CD BioSciences

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Atg4A Antibody

Atg4A Antibody

SPA-00686

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0.1 mg Online Inquiry
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Target Information
Target Name Atg4A
Gene Abbr. ATG4A
Gene ID 115201
Full Name autophagy related 4A cysteine peptidase
Alias APG4A, AUTL2
Introduction Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Control of autophagy was largely discovered in yeast and involves proteins encoded by a set of autophagy-related genes (Atg). Formation of autophagic vesicles requires a pair of essential ubiquitin-like conjugation systems, Atg12-Atg5 and Atg8-phosphatidylethanolamine (Atg8-PE), which are widely conserved in eukaryotes. Numerous mammalian counterparts to yeast Atg proteins have been described, including three Atg8 proteins (GATE-16, GABARAP, and LC3) and four Atg4 homologs (Atg4A/autophagin-2, Atg4B/autophagin-1, Atg4C/autophagin-3, and Atg4D/autophagin-4). The cysteine protease Atg4 is pivotal to autophagosome membrane generation and regulation. Atg4 primes the Atg8 homolog for lipidation by cleaving its carboxy terminus and exposing its glycine residue for E1-like enzyme Atg7. The Atg8 homolog is transferred to the E2-like enzyme Atg3 before forming the Atg8-PE conjugate. During later stages of autophagy, Atg4 can reverse this lipidation event by cleaving PE, thereby recycling the Atg8 homolog.Atg4A predominately cleaves GATE-16, athough it can cleave the other mammalian Atg8 homologues with lesser efficiencies. Mutation in the corresponding Atg4A gene is critical for redox regulation and inhibits autophagosome formation. Expression of this Atg4A mutation demonstrates a role for reactive oxygen species in nutrient-deprived autophagy.
Product Details
Host Sheep
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ATG4A. Glu2-Val398. Accession # Q8WYN0.
Usage
Application WB, IP
Dilutions Western Blot (1 µg/mL); Immunoprecipitation (25 µg/mL)
MW(KDa) 48-60
Reactivity Human
Specificity Detects human ATG4A in direct ELISAs and Western Blots. In direct ELISAs and Western Blots, less than 1% cross-reactivity with recombinant human ATG4GB is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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