ASK1 Antibody - CD BioSciences

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ASK1 Antibody

ASK1 Antibody

SPA-00590

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Target Information
Target Name ASK1
Gene Abbr. MAP3K5
Gene ID 4217
Full Name mitogen-activated protein kinase kinase kinase 5
Alias ASK1, MAPKKK5, MEKK5
Introduction Apoptosis signal-regulating kinase 1 (ASK1), a MAP kinase kinase kinase, plays essential roles in stress-induced apoptosis. ASK1 is activated in response to a variety of stress-related stimuli through distinct mechanisms and activates MKK4 and MKK3, which in turn activate JNK and p38. Overexpression of ASK1 activates JNK and p38 and induces apoptosis in several cell types through signals involving the mitochondrial cell death pathway. Embryonic fibroblasts or primary neurons derived from ASK1-/- mice are resistant to stress-induced JNK and p38 activation as well as cell death. Phosphorylation at Ser967 is essential for ASK1 association with 14-3-3 proteins and suppression of cell death. Oxidative stress induces dephosphorylation of Ser967 and phosphorylation of Thr845 in the activation loop of ASK1, both of which are correlated with ASK1 activity and ASK1-dependent apoptosis. Akt phosphorylates ASK1 at Ser83, which attenuates ASK1 activity and promotes cell survival.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic peptide corresponding to residues surrounding amino acid residue 280 of human ASK1.
Source/Purification Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding amino acid residue 280 of human ASK1. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 155
Reactivity Human
Sensitivity Endogenous
Specificity It detects endogenous ASK1 protein independent of its phosphorylation state.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.