ARA54 Antibody - CD BioSciences

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ARA54 Antibody

ARA54 Antibody

SPA-00552

Size Price
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100 µg Online Inquiry
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Target Information
Target Name ARA54
Gene Abbr. RNF14
Gene ID 9604
Full Name ring finger protein 14
Alias ARA54, HFB30, HRIHFB2038, TRIAD2
Introduction ARA54 (androgen receptor associated protein 54; also RING finger protein 14) is a 50‑55 kDa member of the zinc finger superfamily of proteins. It is ubiquitously expressed, and has two functions; one is to serve as a homodimeric co‑activator for androgen receptor‑induced transcription, and a second as an E3 ubiquitin-protein ligase. Human ARA54 is 474 amino acids (aa) in length and contains an RDW domain that interacts with E2 ubiquitin ligase (aa 11‑137), two zinc finger domains that qualify as RING-type (aa 220‑266) and IBR-type (aa 289‑350), and an androgen-interaction region (aa 361‑474) that contains a coiled‑coil domain. There is one alternate start site at Met127. Over aa 127‑261, human ARA54 is 87% aa identical to mouse ARA54.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Recombinant Protein corresponding to amino acids: GCTMGICSSCNFAFCTLCRLTYHGVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKLDGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEV.
Usage
Application WB, IF, IHC
Dilutions Western Blot (0.04-0.4 µg/mL); Immunofluorescence (0.25-2 µg/mL); Immunohistochemistry (1:20-1:50)
Reactivity Human, Mouse, Rat
Specificity Specificity of human ARA54 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Storage & Handling
Storage Buffer PBS (pH 7.2) and 40% Glycerol.
Preservative 0.02% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.