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AMFR siRNA
SPR-01384
| Size | Price |
| 1 Unit | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | AMFR |
| Gene Abbr. | AMFR |
| Gene ID | 267 |
| Full Name | autocrine motility factor receptor |
| Alias | GP78, RNF45 |
| Introduction | Autocrine motility factor receptor (AMFR/gp78) is a putative seven transmembrane domain G protein-coupled receptor that functions, in part, at the cell surface as a cytokine receptor for autocrine motility factor/phosphoglucose isomerase (AMF/PGI). AMFR is also localized to an intracellular mitochondria-associated smooth ER domain where it functions as an E3 ubiquitin ligase. AMFR function, as both a cytokine receptor and ubiquitin ligase, is linked to a variety of cellular signaling cascades associated with metastasis development and increased invasiveness. AMFR was initially proposed to be a RING-H2 E3 ubiquitin ligase after sequence analysis identified a catalytic RING finger and CUE motif, which are responsible for ubiquitin ligase activity and ubiquitin binding, respectively. Indeed, AMFR is a key component and amongst the best characterized ubiquitin ligases of the endoplasmic reticulum associated degradation (ERAD) machinery, a process involving recognition of misfolded proteins, ubiquitination, deglycosylation, retro-translocation to the cytosol, and targeting to the proteasome. Recent studies have shown that AMFR plays an important role in cholesterol homeostasis via the sterol-mediated ubiquitination of HMG-CoA reductase and its cofactor Insig-1. Furthermore, AMFR has been implicated in the degradation of apolipoprotein B100. It was recently reported that AMFR degrades the metastasis suppressor KAI-1/CD-82, representing the first evidence that AMFR ubiquitin ligase activity is involved in metastasis development. Increased expression of AMFR correlates with a high incidence of recurrence and reduced survival in patients with bladder, colorectal, and gastric cancers. |
| Usage | |
|---|---|
| Application | RNA interference |
| Reactivity | |
| Storage & Handling | |
|---|---|
| Storage Buffer | The siRNA product is supplied in RNAse-free water. |
| Storage Temp. | Aliquot and store at -20 °C. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.