ALKBH2 Knockout Cell Line - CD BioSciences

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ALKBH2 Knockout Cell Line

ALKBH2 Knockout Cell Line

SPL-00216

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Description
7bp deletion
Target Information
Target Name ALKBH2
Gene Abbr. ALKBH2
Gene ID 121642
Full Name alkB homolog 2, alpha-ketoglutarate dependent dioxygenase
Alias ABH2
Species Human
Genomic Locus chr12:109092603
Transcript NM_001145375
WT Expression Level 78.68 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in single-stranded DNA. ALKBH2 and ALKBH3 (MIM 610603) are E. coli AlkB homologs that catalyze the removal of 1-methyladenine and 3-methylcytosine (Duncan et al., 2002 [PubMed 12486230]).[supplied by OMIM, Mar 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 7bp deletion in a coding exon of ALKBH2.
Description 7bp deletion
Parental Cell Line C631
Guide RNA Sequence CCCGAATGTGCCGCCAGCTA
PCR Primer Forward: TCCTGAGTTCAGATTTCTCCAAACA
Reverse: GATGGACAGATTCCTGGTGAAAGG
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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