ALKBH1 Knockout Cell Line - CD BioSciences

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ALKBH1 Knockout Cell Line

ALKBH1 Knockout Cell Line

SPL-00214

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Description
16bp deletion
Target Information
Target Name ALKBH1
Gene Abbr. ALKBH1
Gene ID 8846
Full Name alkB homolog 1, histone H2A dioxygenase
Alias ABH, ABH1, ALKBH, alkB, hABH
Species Human
Genomic Locus chr14:77704400
Transcript NM_006020
WT Expression Level 14.32 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 16bp deletion in a coding exon of ALKBH1.
Description 16bp deletion
Parental Cell Line C631
Guide RNA Sequence GTCTTCAGCCCGTCAGCAAG
PCR Primer Forward: GACAAGGTACAGTCTAGAATGGGTT
Reverse: GCCTCATACAAGAAATGTGTTTGGA
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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