AKT3 Knockout Cell Line - CD BioSciences

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AKT3 Knockout Cell Line

AKT3 Knockout Cell Line

SPL-00199

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Description
100bp insertion
Target Information
Target Name Akt
Gene Abbr. AKT3
Gene ID 10000
Full Name AKT serine/threonine kinase 3
Alias MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG
Species Human
Genomic Locus chr1:243695617
Transcript NM_001206729
WT Expression Level 26.64 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 100bp insertion in a coding exon of AKT3.
Description 100bp insertion
Parental Cell Line C631
Guide RNA Sequence TAAGGTAAATCCACATCTTG
PCR Primer Forward: AACCCAAACTTTTTCACAGGTTTCT
Reverse: GGCCAAGATACTTCCTTTTGAAGAC
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

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