AKT1S1 Knockout Cell Line - CD BioSciences

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AKT1S1 Knockout Cell Line

AKT1S1 Knockout Cell Line

SPL-00192

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1 Unit Online Inquiry
Description
56bp deletion
Target Information
Target Name AKT1S1
Gene Abbr. AKT1S1
Gene ID 84335
Full Name AKT1 substrate 1
Alias Lobe, PRAS40
Species Human
Genomic Locus chr19:49873220
Transcript NM_032375
WT Expression Level 36.83 TPM (TPM = Transcripts per million; any value less than 3 is considered non-expressing)
Introduction AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008].
Product Details
Cell Line Model HAP1
Genotype HAP1 cell line, edited by CRISPR/Cas to contain a 56bp deletion in a coding exon of AKT1S1.
Description 56bp deletion
Parental Cell Line C631
Guide RNA Sequence CTTCCGGGCCCGGACTGGCA
PCR Primer Forward: TCTCACCTCCATTATCACTAATGCC
Reverse: AAAGGGGAAGGAGTACAGTGACTT
Handling Specifications
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into 10 mL of pre-warmed media in a 10 cm dish.
Culture Medium IMDM + 10% FCS
Growth Properties Cells are adherent cells that are cultured at 37°C in a humidified atmosphere with 5% CO2. Cells should be passaged every 2-3 days, splitting approximately 1:10-1:20.
Freeze Medium IMDM + 20% FCS + 10% DMSO
Biosafety Level BSL-1
Disclaimer This product is classified under IATA regulations as a GMMO (genetically modified micro-organism) and will ship as UN3245. If applicable, ensure facility meets all requirements per local and country regulations.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.